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41.
42.
光照强度对海洋微藻脂肪含量及脂肪酸组成影响的研究   总被引:1,自引:0,他引:1  
近年来,海洋微藻脂肪酸组成的研究及其应用越来越受到国内外科学家的重视。高度不饱和脂肪酸(PUFA),特别是长链的n-3 PUFA,如二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)对海洋动物和人类都具有营养学和医学上的价值。在海洋微藻的培养过程中,光照强度是海洋微藻生长的条件之一,光线的明与暗,光度的强与弱,不仅对微藻的生长速率、产量有影响,而且对其脂肪含量和脂肪酸的组成也有影响(Teshima et al.,1983;Thompson et al.,1990; Renaud et al., 1991)。 作者在以往研究的基础上,选择了3种有代表性的海洋微藻:(1)小球藻Chlorella sp-2(李荷芳等,1999),此藻脂肪酸中EPA含量高、且不含DHA;(2)球等鞭金藻(Isochrysis galbana),该藻DHA含量较高,但几乎不含EPA;(3)前沟藻(Amphidinium sp.)的EPA、DHA含量均高。将以上3种微藻作为原料,在不同的光照强度下进行培养,测定并分析藻体中的脂肪含量和脂肪酸组成的变化,从而了解光强对海洋微藻脂肪含量及其脂肪酸组成的影响。  相似文献   
43.
鱼类生长激素的异源表达、应用及安全性评价   总被引:3,自引:0,他引:3  
鱼类生长激素对鱼的生长发育起着重要的调节作用,能促进鱼体快速生长,提高饵料转化率,在水产养殖业中具有重大应用价值。转生长激素基因鱼具有快速生长效应,可以缩短养殖周期,降低养殖成本和风险。鱼类生长激素基因异源表达体系的建立和发展,使获得大量廉价的鱼类生长激素产品成为可能,为鱼类养殖业新型饵料添加剂的研制开辟了新的途径。本文综述了转生长激素基因鱼和鱼类生长激素基因的异源表达两方面的研究及其安全性评价。  相似文献   
44.
A new method is presented to process and correct full-depth current velocity data obtained from a lowered acoustic Doppler current profiler (LADCP). The analysis shows that, except near the surface, the echo intensity of a reflected sound pulse is closely correlated with the magnitude of the difference in vertical shear of velocity between downcast and upcast, indicating an error in velocity shear. The present method features the use of echo intensity for the correction of velocity shear. The correction values are determined as to fit LADCP velocity to shipboard ADCP (SADCP) and LADCP bottom-tracked velocities. The method is as follows. Initially, a profile of velocity relative to the sea surface is obtained by integrating vertical shears of velocity after low-quality data are rejected. Second, the relative velocity is fitted to the velocity at 100–800 dbar measured by SADCP to obtain an “absolute” velocity profile. Third, the velocity shear is corrected using the relationship between the errors in velocity shears and echo intensity, in order to adjust the velocity at sea bottom to the bottom-tracked velocity measured by LADCP. Finally, the velocity profile is obtained from the SADCP-fitted velocity at depths less than 800 dbar and the corrected velocity shear at depths greater than 800 dbar. This method is valid for a full-depth LADCP cast throughout which the echo intensity is relatively high (greater than 75 dB in the present analysis). Although the processed velocity may include errors of 1–2 cm s−1, this method produced qualitatively good current structures in the Northeast Pacific Basin that were consistent with the deep current structures inferred from silicate distribution, and the averaged velocities were significantly different from those calculated by the Visbeck (2002) method.  相似文献   
45.
从野外采集鹤鸪菜Caloglossaleprieurii(Mont.)J.Ag.幼体,在室内控制条件下,以温、盐、光等不同条件进行模拟培养试验。所获得的数据经生物统计检验表明:鹧鸪菜幼体生长最适水温为23℃;光照强度为4000lx;海水盐度为18。此结果与我们多年来在汕头港内观察鹧鸪菜的生长及其季节变化相似。  相似文献   
46.
海洋生物发光研究对渔业、环境监测等有着举足轻重的意义。根据从湛江东海岛海域沉积物样品中分离出的一株海洋发光弧菌(Vibrio sp.,编号为D2),对这株菌的生长和发光条件进行了初步研究。实验结果表明,海洋发光弧菌D2在pH7.0、温度35℃、NaCl浓度为2.0%时,生长状态最好;在pH5~6、温度20℃、NaCl浓度为3.0%、细菌密度OD600达0.08时,发光强度最高。  相似文献   
47.
不同环境下镉对小球藻吸收磷速率的影响   总被引:1,自引:0,他引:1  
在人工配制的污水中进行静态模拟实验,研究了不同光照强度及不同pH值下,镉对小球藻(Chlorella vulgaris)在悬浮和固定状态下对污水中正磷酸盐的吸收速率的影响。结果表明,镉存在的情况下,在实验所设的各光照强度内,悬浮态小球藻对磷的吸收速率为4~10μg/(h.个),固定化小球藻为12~16μg/(h.个);而相同的镉浓度下,在不同的pH范围中,悬浮态小球藻对磷的吸收速率为1.9~9.4μg/(h.个),固定化小球藻为5.7~12μg/(h.个)。在不同光照强度下,镉总体上降低了小球藻对磷的吸收速率,其中使悬浮藻普遍下降40%~60%,而固定处理使藻受镉的影响较少,至多下降10%~20%,但在个别光照强度下镉反而促进了小球藻对磷的吸收速率。不同pH值时,无论悬浮态还是固定态,镉在大多pH下使小球藻的磷吸收速率下降20%~30%,固定处理并没有减少镉的影响,同样在个别pH下镉促进了对磷的吸收。  相似文献   
48.
In this article a method to estimate the mean upcrossing intensity, μ(u), of a stochastic process is proposed. It is assumed that the stochastic process is a sum of a Gaussian process and a second-order correction term. The method is based on the two-dimensional Saddlepoint approximation. The accuracy of the method is tested on processes having analytical solutions for μ(u). Numerical examples are given where the stochastic process represents (i) the horizontal response of a floating offshore structure in a Gaussian sea, and (ii) the response of a structure subjected to a Gaussian wind velocity process. In addition, the estimates are compared to empirical upcrossing intensities of simulated responses. For case (ii), the obtained μ(u) estimates are compared to estimates obtained by numerical integration.  相似文献   
49.
Chesapeake Bay is a large and productive estuary that has received close scrutiny in recent years because of indications that its water quality and biota have been damaged by man's activities. Data on primary production for the estuary as a whole, however, are surprisingly sparse. We describe here the distribution of photosynthetic carbon assimilation by phytoplankton in Chesapeake Bay, and relate productivity patterns to hydrographic characteristics of the estuary. Between March 1982 and April 1983, a series of four cruises was conducted on Chesapeake Bay, and two cruises on the urbanized Delaware Bay for comparison. The upper Chesapeake and Delaware were highly turbid with high concentrations of suspended particulate matter and dissolved inorganic nutrients. Low chlorophyll concentrations were usually found in these areas of high turbidity, despite the abundance of nutrients, suggesting light limitation. Application of Wofsy's (1983) model of phytoplanton growth confirmed this suggestion. Chlorophyll and productivity maxima usually occurred seaward of the turbidity maxima where light penetration increased and suffient nutrients were present to support active phytoplankton growth. Further seaward of the chlorophyll maxima in the Chesapeake, the photic zone depth increased, concentrations of nutrients decreased, and phytoplankton biomass decreased, suggesting that nutrient availability, rather than light, controlled phytoplankton growth in the lower portion of the estuary. In contrast to the Chesapeake, Delaware Bay was more turbid, had generally higher nutrient concentrations, and was lower in phytoplankton productivity. The chlorophyll maxima and region of rapid phytoplankton growth occurred further toward the lower estuary and shelf regions in Delaware Bay because the high turbidity extended further seaward. Nutrients were never depleted at the shelf end of the estuary sufficiently to retard phytoplankton growth. Photosynthesis-irradiance (P-I) curves from simulated in situ and constant intensity incubations showed a strong correlation of the light-limited slope (aB) with the light-saturated rate ( ) on each cruise. Spatial variations in corresponded to patterns of phytoplankton abundance, as did integral production (PP) and carbon-based growth rates (μC, μm), and photosynthetic parameters varied significantly with temperature.  相似文献   
50.
Offshore petroleum drilling wastes contain toxic substances that are potentially harmful to the marine ecosystem. Despite environmentally benign characteristics, wastes associated with synthetic-based fluids still contain a certain amount of pollutants due to contamination with formation oil and the presence of trace heavy metals in barite, which may pose risk when discharged into the marine environment. A framework is presented here for a decision support system for the selection of the best drilling waste discharge option. Uncertainties in the quantification of risk, cost and technical feasibility are expressed by fuzzy numbers. An analytical hierarchy process with a technique called fuzzy synthetic evaluation is employed to determine the best management alternative (discharge scenario).  相似文献   
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